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SYBR Green Real-Time PCR Master Mixes
SYBR™ Green master mixes are designed for quantitative real-time PCR using a set of two PCR primers that flank the target region. The master mixes contain buffer, dNTPs, thermostable hot-start DNA polymerase, and of course, SYBR Green dye—everything needed for real-time PCR, except the sample and PCR primer pair.
SYBR Green Master Mix formulations have improved over the years, as researchers have demanded improved sensitivity, greater specificity, enhanced reproducibility, faster time-to-results, and protection from contamination. Although there are now many choices of SYBR Green master mixes available, below are the recommended master mixes, by application.
| Applications | Gene expression analysis (Microbe detection, ChIP DNA analysis) | Gene expression analysis (Microbe detection, ChIP DNA analysis) | Genotyping by high resolution melt (HRM) analysis (Mutation scanning Methylation analysis) |
| Recommended master mix | PowerUp™ SYBR™ Green Master Mix | Power SYBR™ Green Master Mix | MeltDoctor™ HRM Master Mix |
| Performance | Best | Better | Best |
| Hot start | Dual-lock for exceptional specificity
|  | |
| Cycling mode | Standard (~60 min) or Fast (~30 min) | Standard (~60 min) | Standard (~70 min) |
| Carryover contamination control | Includes heat-labile UNG and a blend of dTTP/dUTP | Contains dUTP, which is compatible with AmpErase™ UNG (available separately) | Contains dUTP, which is compatible with AmpErase UNG (available separately) |
| 72-hour stability of reaction mixes prior to qPCR | | ||
| Instrument compatibility | Compatible with all Applied Biosystems™ real-time PCR instruments, Bio-Rad iQ™5 and CFX™ (96 and 384), Roche LightCycler™ 480, and Stratagene™ Mx3005P™ systems | Compatible with all Applied Biosystems real-time PCR instruments, Bio-Rad iQ5, Roche LightCycler 480, and Stratagene Mx3005P systems | Compatible with Applied Biosystems real-time PCR instruments running High Resolution Melt Software |
| Recommended primer concentration | 300–800 nM | 50–900 nM | 50–900 nM |
See the entire range of SYBR Green master mixes available from Thermo Fisher Scientific below.
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Comments | |
| Applied Biosystems™ products | Wide instrument compatibility, and the best choice for Applied Biosystems instruments. All mixes include ROX™ dye. | ||||||||
| PowerUp SYBR Green Master Mix (Recommended) |  | | | |  | | | Our newest and best mix. Formulated for exceptional specificity, single-copy detection, and wide instrument compatibility. | |
| Power SYBR Green Master Mix (Best value) | | | Our most popular mix. A great value. | ||||||
| SYBR Select Master Mix | | | | | | Our brightest mix. May be too bright for some older instruments. | |||
| Fast SYBR Green Master Mix | | | | | Our first mix formulated for fast cycling. | ||||
| SYBR Green Master Mix | | | The original Applied Biosystems™ SYBR Green master mix. | ||||||
| MeltDoctor HRM Master Mix | | Formulated specifically for high resolution melt (HRM) analysis. | |||||||
| Thermo Scientific™ products | Mixes optimized for specific instruments from Thermo Fisher Scientific and other suppliers. Versions with separate ROX, low ROX, high ROX, and fluorescein available. | ||||||||
| Luminaris™ Color HiGreen qPCR Master Mix | | | |  | | Includes dye in mix and in sample buffer to aid pipetting. | |||
| Maxima™ SYBR Green qPCR Master Mix | | | A basic mix with great value, especially if you don’t want premixed ROX. | ||||||
| DyNAmo™ ColorFlash SYBR Green qPCR Kit | | | | | | A fast cycling–compatible mix that includes dye in mix and in sample buffer to aid pipetting. | |||
| Absolute™ QPCR Mix, SYBR Green | | A basic mix, and the only one available without dUTP. | |||||||
| Invitrogen™ products | Mixes optimized for specific instruments from Thermo Fisher Scientific and other suppliers. Versions with separate ROX, low ROX, high ROX, and fluorescein available. | ||||||||
| SYBR™ GreenER™ qPCR SuperMix | | | | An updated, brighter version of Platinum™ SYBR Green supermix. | |||||
| Express SYBR™ GreenER™ qPCR SuperMix | | | | | All the features of SYBR GreenER mix, but fast cycling–compatible. To be discontinued. | ||||
| Platinum™ SYBR™ Green qPCR SuperMix-UDG | | | | The original Invitrogen SYBR Green master mix. | |||||
1. • = master mix includes uracil N-glycosylase (UNG); •• = master mix includes a heat-labile form of UNG, which improves master mix performance compared to the standard UNG.
2. Master mix includes ROX, a passive reference dye; V = varies, different master mix versions are available with different amounts of ROX dye, or ROX included in a separate tube.
